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plasmids pcdna3 1 ms2ha hssmg5 t  (Addgene inc)


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    Structured Review

    Addgene inc plasmids pcdna3 1 ms2ha hssmg5 t
    Plasmids Pcdna3 1 Ms2ha Hssmg5 T, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmids+pcdna3+1+ms2+ha+hssmg5+t/pcDNA3%2E1-MS2-HA-HsSmg5_T+(Plasmid+%23147552)/pm41763597-198-32-34
    Average 92 stars, based on 5 article reviews
    plasmids pcdna3 1 ms2ha hssmg5 t - by Bioz Stars, 2026-10
    92/100 stars

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    Amplification:

    Article Title: Active site assembly by SMG5 as a mechanism for SMG6 endonuclease licensing in nonsense-mediated mRNA decay
    Article Snippet: .. Human SMG5 and SMG6 sequences were amplified by PCR from plasmids pcDNA3.1-MS2-HA-HsSmg5_T (Addgene #147552) [ ] and pCIneo-λN-HA-HsSmg6_I (Addgene #146551) [ ], or from HEK293 cell cDNA, and then cloned by Golden-gate cloning into low-copy plasmid pLIBT7 in fusion with N-terminal His-tag (for SMG5), N-terminal HisGST-tag (for SMG6), N-terminal SpyTag [ ] and C-terminal His-tag (for SMG5) or N-terminal SpyCatcher [ ] and C-terminal His-tag (for SMG6). ..

    Polymerase Chain Reaction:

    Article Title: Active site assembly by SMG5 as a mechanism for SMG6 endonuclease licensing in nonsense-mediated mRNA decay
    Article Snippet: .. Human SMG5 and SMG6 sequences were amplified by PCR from plasmids pcDNA3.1-MS2-HA-HsSmg5_T (Addgene #147552) [ ] and pCIneo-λN-HA-HsSmg6_I (Addgene #146551) [ ], or from HEK293 cell cDNA, and then cloned by Golden-gate cloning into low-copy plasmid pLIBT7 in fusion with N-terminal His-tag (for SMG5), N-terminal HisGST-tag (for SMG6), N-terminal SpyTag [ ] and C-terminal His-tag (for SMG5) or N-terminal SpyCatcher [ ] and C-terminal His-tag (for SMG6). ..

    Clone Assay:

    Article Title: Active site assembly by SMG5 as a mechanism for SMG6 endonuclease licensing in nonsense-mediated mRNA decay
    Article Snippet: .. Human SMG5 and SMG6 sequences were amplified by PCR from plasmids pcDNA3.1-MS2-HA-HsSmg5_T (Addgene #147552) [ ] and pCIneo-λN-HA-HsSmg6_I (Addgene #146551) [ ], or from HEK293 cell cDNA, and then cloned by Golden-gate cloning into low-copy plasmid pLIBT7 in fusion with N-terminal His-tag (for SMG5), N-terminal HisGST-tag (for SMG6), N-terminal SpyTag [ ] and C-terminal His-tag (for SMG5) or N-terminal SpyCatcher [ ] and C-terminal His-tag (for SMG6). ..

    Cloning:

    Article Title: Active site assembly by SMG5 as a mechanism for SMG6 endonuclease licensing in nonsense-mediated mRNA decay
    Article Snippet: .. Human SMG5 and SMG6 sequences were amplified by PCR from plasmids pcDNA3.1-MS2-HA-HsSmg5_T (Addgene #147552) [ ] and pCIneo-λN-HA-HsSmg6_I (Addgene #146551) [ ], or from HEK293 cell cDNA, and then cloned by Golden-gate cloning into low-copy plasmid pLIBT7 in fusion with N-terminal His-tag (for SMG5), N-terminal HisGST-tag (for SMG6), N-terminal SpyTag [ ] and C-terminal His-tag (for SMG5) or N-terminal SpyCatcher [ ] and C-terminal His-tag (for SMG6). ..

    Plasmid Preparation:

    Article Title: Active site assembly by SMG5 as a mechanism for SMG6 endonuclease licensing in nonsense-mediated mRNA decay
    Article Snippet: .. Human SMG5 and SMG6 sequences were amplified by PCR from plasmids pcDNA3.1-MS2-HA-HsSmg5_T (Addgene #147552) [ ] and pCIneo-λN-HA-HsSmg6_I (Addgene #146551) [ ], or from HEK293 cell cDNA, and then cloned by Golden-gate cloning into low-copy plasmid pLIBT7 in fusion with N-terminal His-tag (for SMG5), N-terminal HisGST-tag (for SMG6), N-terminal SpyTag [ ] and C-terminal His-tag (for SMG5) or N-terminal SpyCatcher [ ] and C-terminal His-tag (for SMG6). ..



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